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http://ir.ncue.edu.tw/ir/handle/987654321/11506
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| Title: | Analysis of an SMN Gene-like DNA Fragment
SMN類似基因的分析 |
| Authors: | Jong, Yuh-Jyh;Li, Hung;Chang, Jan-Gowth;Hsieh-Li, Hsiu-Mei;Lee, Hsien-Hsiung;Lee, Cheng-Chun;Wang, Nancy M.;Tsai, Chang-Hai |
| Contributors: | 生物技術研究所 |
| Keywords: | Spinal muscular atrophy;Survival motor neuron gene;Pseudogene |
| Date: | 2000-03
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| Issue Date: | 2012-06-06T02:04:04Z
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| Publisher: | 高雄醫學大學 |
| Abstract: | Part of a survivial motor neuron (SMN) gene-like DNA fragment has been identified .This DNA fragment was accidentally isolated from cDNA by RT–PCR using primers specific for the region between exon 3 and 6 of the SMN gene .This fragment was used as a probe to hybridize the mRNA from several tissues, but we have been unable to detect any transcript of this SMN-like gene in these tissues. Thus, we have inferred this SMN gene-like fragment was a genomic product contaminant that was amplified in the reaction . Sequencing analysis of this fragment , which contains several stop codons, revealed a 74.6% nucleotide homology with the SMN gene. From these results, we believe that this DNA fragment is not a mutated form of SMN gene . Rather , it is an SMN-like pseudogene ,which is variably present even in normal individuals.
我們利用RT–PCR方法分析SMN基因的表現，分析其產物偶然發現一段與SMN基因非常相似的產物，其DNA序列的分析結果顯示有74.6%的核?酸同質性，但其中含有幾個停止的密碼，我們又發現此基因片斷在各種組織並無表現，因此，我們認為它是一個SMN偽基因的片斷。此偽基因片斷並無intron，而且有些正常人並無此基因。 |
| Relation: | The Kaohsiung Journal of Medical Sciences, 16(3): 112-116 |
| Appears in Collections: | [生物技術研究所] 期刊論文
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