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Title: Analysis of an SMN Gene-like DNA Fragment
Authors: Jong, Yuh-Jyh;Li, Hung;Chang, Jan-Gowth;Hsieh-Li, Hsiu-Mei;Lee, Hsien-Hsiung;Lee, Cheng-Chun;Wang, Nancy M.;Tsai, Chang-Hai
Contributors: 生物技術研究所
Keywords: Spinal muscular atrophy;Survival motor neuron gene;Pseudogene
Date: 2000-03
Issue Date: 2012-06-06T02:04:04Z
Publisher: 高雄醫學大學
Abstract: Part of a survivial motor neuron (SMN) gene-like DNA fragment has been identified .This DNA fragment was accidentally isolated from cDNA by RT–PCR using primers specific for the region between exon 3 and 6 of the SMN gene .This fragment was used as a probe to hybridize the mRNA from several tissues, but we have been unable to detect any transcript of this SMN-like gene in these tissues. Thus, we have inferred this SMN gene-like fragment was a genomic product contaminant that was amplified in the reaction . Sequencing analysis of this fragment , which contains several stop codons, revealed a 74.6% nucleotide homology with the SMN gene. From these results, we believe that this DNA fragment is not a mutated form of SMN gene . Rather , it is an SMN-like pseudogene ,which is variably present even in normal individuals.
Relation: The Kaohsiung Journal of Medical Sciences, 16(3): 112-116
Appears in Collections:[biotech] Periodical Articles

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