National Changhua University of Education Institutional Repository : Item 987654321/11513
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6507/11669
Visitors : 29734025      Online Users : 506
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister
NCUEIR > College of Science > biotech > Periodical Articles >  Item 987654321/11513

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/11513

Title: Expression of the American Cockroach Per a 1 Allergen in Mammalian Cells
Authors: Wu, C. H.;Lee, M. F.;Wang, Nancy M.
Contributors: 生物技術研究所
Keywords: American cockroach;Cross-reactive allergen;Expression in mammalian cells;Per a 1 allergen
Date: 2000-12
Issue Date: 2012-06-06T02:04:37Z
Publisher: John Wiley & Sons, Inc.
Abstract: BACKGROUND: Cockroach allergens are one of the major etiologic risk factors for developing IgE-mediated allergic respiratory illness throughout the world. Per a 1 is a cross-reactive allergen of American and German cockroaches. This study aimed to investigate the expression of a recombinant American cockroach (Periplaneta americana) Per a 1, C42, allergen in mammalian COS-1 cells. METHODS: The COS-1 cells and Escherichia coli were used to express the P. americana C42 allergen. Recombinant proteins were purified with hydroxylapatite and DE52 chromatography. Biologic reactivities of recombinant proteins were examined by direct IgE binding and IgE inhibition studies with the enzyme-linked immunosorbent assay (ELISA). RESULTS: C42 was successfully expressed in the mammalian COS-1 cell as a 50-kDa secreted protein, and purified from the culture medium. The specific human IgE antibodies against recombinant C42 from either E. coli (C42-E. coli) or COS-1 (C42-COS-1) were compared by ELISA with 12 sera from Per a 1 and C42 skin-test-positive patients. All atopic sera contained specific IgE antibodies to C42 from either E. coli or COS-1. Moreover, recombinant C42-COS-1 bound higher levels of serum IgE than recombinant C42-E. coli among C42-sensitive atopic patients, and a statistically significant difference (P<0.01) was found between them. In addition, recombinant C42-COS-1 as an inhibitor revealed higher inhibition of IgE binding to natural Per a 1 than recombinant C42-E. coli. CONCLUSIONS: The biologically highly reactive recombinant C42 produced in the COS-1 cell provides an alternative expression system and will facilitate studies on the immune response of asthma patients to cockroach allergens.
Relation: Allergy., 55(12): 1179-1183
Appears in Collections:[biotech] Periodical Articles

Files in This Item:

File SizeFormat
index.html0KbHTML663View/Open


All items in NCUEIR are protected by copyright, with all rights reserved.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback