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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/11698

Title: Sequence based prediction of protein mutant stability and discrimination of thermophilic proteins
Authors: Gromiha, M. M.;Huang, Liang-Tsung;Lai, Lien-Fu
Contributors: 資訊工程學系
Keywords: Protein stability;Rule generator;Discrimination;Prediction;Thermophilic proteins;Neural network;Machine learning techniques
Date: 2008-10
Issue Date: 2012-07-02T02:02:15Z
Publisher: Springer
Abstract: Prediction of protein stability upon amino acid substitution and discrimination of thermophilic proteins from mesophilic ones are important problems in designing stable proteins. We have developed a classification rule generator using the information about wild-type, mutant, three neighboring residues and experimentally observed stability data. Utilizing the rules, we have developed a method based on decision tree for discriminating the stabilizing and destabilizing mutants and predicting protein stability changes upon single point mutations, which showed an accuracy of 82% and a correlation of 0.70, respectively. In addition, we have systematically analyzed the characteristic features of amino acid residues in 3075 mesophilic and 1609 thermophilic proteins belonging to 9 and 15 families, respectively, and developed methods for discriminating them. The method based on neural network could discrimi-nate them at the 5-fold cross-validation accuracy of 89% in a dataset of 4684 proteins and 91% in a test set of 707 proteins.
Relation: Lectures Notes in Bioinformatics (LNBI 5265): 1-12
Appears in Collections:[資訊工程學系] 期刊論文

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