National Changhua University of Education Institutional Repository : Item 987654321/11708
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题名: Development of knowledge based system for predicting the stability of proteins upon point mutations
作者: Huang, Liang-Tsung;Lai, Lien-Fu;Wu, Chao-Chin;Gromiha, M. Michael
贡献者: 資訊工程學系
日期: 2010-08
上传时间: 2012-07-02T02:03:31Z
出版者: Elsevier Science Publishers B. V.
摘要: Prediction of protein stability upon amino acid substitution is an important problem in designing stable proteins. We have developed a classification rule generator for integrating the knowledge of amino acid sequence and experimental stability change upon single mutation. These rules are human readable and hence the method enhances the synergy between expert knowledge and computational system. Utilizing the information about wild type, mutant, three neighboring residues and experimentally observed stability data, we have developed a method based on decision tree for discriminating the stabilizing and destabilizing mutants and predicting the protein stability changes upon single point mutations, which showed an accuracy of 82% and a correlation of 0.70, respectively. In addition, we have developed a fuzzy query method to predict protein stability with partial information. We have developed a web server for predicting the protein stability changes upon single mutations by using fuzzy query mechanism and it is available at http://bioinformatics.myweb.hinet.net/fqstab.htm.
關聯: Neurocomputing, 73(13-15): 2293-2299
显示于类别:[資訊工程學系] 期刊論文

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