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NCUEIR > College of Engineering > Department and Graduate Institute of Computer Science and Information Engineering > Periodical Articles > Item 987654321/11709

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/11709

Title:	Predicting Protein Stability Change upon Double Mutation from Partial Sequence Information Using Data Mining Approach
Authors:	Lai, Lien-Fu;Wu, Chao-Chin;Huang, L. T.
Contributors:	資訊工程學系
Keywords:	Data mining;Double mutation;Fuzzy query;Protein stability;Sequence information
Date:	2010-08
Issue Date:	2012-07-02T02:03:33Z
Publisher:	Springer Verlag
Abstract:	Prediction of protein stability upon amino acid substitution is an important problem in designing stable proteins. We have developed a classification rule generator for integrating the knowledge of amino acid sequence and experimental stability change upon single mutation. These rules are human readable and hence the method enhances the synergy between expert knowledge and computational system. Utilizing the information about wild type, mutant, three neighboring residues and experimentally observed stability data, we have developed a method based on decision tree for discriminating the stabilizing and destabilizing mutants and predicting the protein stability changes upon single point mutations, which showed an accuracy of 82% and a correlation of 0.70, respectively. In addition, we have developed a fuzzy query method to predict protein stability with partial information. We have developed a web server for predicting the protein stability changes upon single mutations by using fuzzy query mechanism and it is available at http://bioinformatics.myweb.hinet.net/fqstab.htm.
Relation:	Lecture Notes in Computer Science (LNCS 6215): 664-671
Appears in Collections:	[Department and Graduate Institute of Computer Science and Information Engineering] Periodical Articles

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