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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/11932

Title: Fiber-optic multiphoton flow cytometry in whole blood and in vivo
Authors: Chang, Yu-Chung;Ye, Jing Yong;Thommey P. Thomas;Zhengyi Cao;Alina Kotlyar;Eric R. Tkaczyk;James R. Baker Jr.
Contributors: 電機工程學系
Keywords: Two-photon fluorescence;Fiber probe;Double-clad fiber;In vivo flow cytometry;Circulating tumor cell;Cancer;Metastasis;Green fluorescent protein.
Date: 2010-07
Issue Date: 2012-07-03T01:48:45Z
Publisher: SPIE
Abstract: Circulating tumor cells in the bloodstream are sensitive indicators for metastasis and disease prognosis. Circulating cells have usually been monitored via extraction from blood, and more recently in vivo using free-space optics; however, long-term intravital monitoring of rare circulating cells remains a major challenge. We demonstrate the application of a two-photon-fluorescence optical fiber probe for the detection of cells in whole blood and in vivo. A double-clad fiber was used to enhance the detection sensitivity. Two-channel detection was employed to enable simultaneous measurement of multiple fluorescent markers. Because the fiber probe circumvents scattering and absorption from whole blood, the detected signal strength from fluorescent cells was found to be similar in phosphate-buffered saline (PBS) and in whole blood. The detection efficiency of cells labeled with the membrane-binding dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindoldicarbocyanine, 4-chlorobenzenesulfonate (DiD) was demonstrated to be the same in PBS and in whole blood. A high detection efficiency of green fluorescent protein (GFP)–expressing cells in whole blood was also demonstrated. To characterize in vivo detection, DiD-labeled untransfected and GFP-transfected cells were injected into live mice, and the cell circulation dynamics was monitored in real time. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed ex vivo in whole blood.
Relation: Journal of Biomedical Optics, 15(4): 047004
Appears in Collections:[電機工程學系] 期刊論文

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