National Changhua University of Education Institutional Repository : Item 987654321/11932
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6507/11669
造访人次 : 30029746      在线人数 : 602
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/11932

题名: Fiber-optic multiphoton flow cytometry in whole blood and in vivo
作者: Chang, Yu-Chung;Ye, Jing Yong;Thommey P. Thomas;Zhengyi Cao;Alina Kotlyar;Eric R. Tkaczyk;James R. Baker Jr.
贡献者: 電機工程學系
关键词: Two-photon fluorescence;Fiber probe;Double-clad fiber;In vivo flow cytometry;Circulating tumor cell;Cancer;Metastasis;Green fluorescent protein.
日期: 2010-07
上传时间: 2012-07-03T01:48:45Z
出版者: SPIE
摘要: Circulating tumor cells in the bloodstream are sensitive indicators for metastasis and disease prognosis. Circulating cells have usually been monitored via extraction from blood, and more recently in vivo using free-space optics; however, long-term intravital monitoring of rare circulating cells remains a major challenge. We demonstrate the application of a two-photon-fluorescence optical fiber probe for the detection of cells in whole blood and in vivo. A double-clad fiber was used to enhance the detection sensitivity. Two-channel detection was employed to enable simultaneous measurement of multiple fluorescent markers. Because the fiber probe circumvents scattering and absorption from whole blood, the detected signal strength from fluorescent cells was found to be similar in phosphate-buffered saline (PBS) and in whole blood. The detection efficiency of cells labeled with the membrane-binding dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindoldicarbocyanine, 4-chlorobenzenesulfonate (DiD) was demonstrated to be the same in PBS and in whole blood. A high detection efficiency of green fluorescent protein (GFP)–expressing cells in whole blood was also demonstrated. To characterize in vivo detection, DiD-labeled untransfected and GFP-transfected cells were injected into live mice, and the cell circulation dynamics was monitored in real time. The detection efficiency of GFP-expressing cells in vivo was consistent with that observed ex vivo in whole blood.
關聯: Journal of Biomedical Optics, 15(4): 047004
显示于类别:[電機工程學系] 期刊論文

文件中的档案:

档案 大小格式浏览次数
index.html0KbHTML683检视/开启


在NCUEIR中所有的数据项都受到原著作权保护.

 

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈