National Changhua University of Education Institutional Repository : Item 987654321/11933
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题名: Optical Fiber-Based In Vivo Quantification of Growth Factor Receptors
作者: Thommey P. Thomas;Chang, Yu-Chung;Ye, Jing Yong;Kotlyar, Alina;Cao, Zhengyi;Shukla, Rameshwer;Qin, Suyang;Theodore B. Norris;James R. Baker
贡献者: 電機工程學系
关键词: 2-photon excitation;Cancer targeting;Double-clad optical fiber;Growth factor receptor quantification;HER2;Herceptin
日期: 2012-04
上传时间: 2012-07-03T01:48:47Z
出版者: John Wiley & Sons Inc
摘要: BACKGROUND: Growth factor receptors such as epidermal growth factor receptor 1 and human epidermal growth receptor 2 (HER2) are overexpressed in certain cancer cells. Antibodies against these receptors (eg. cetuximab and transtuzumab [Herceptin]) have shown therapeutic value in cancer treatment. The existing methods for the quantification of these receptors in tumors involve immunohistochemistry or DNA quantification, both in extracted tissue samples. The goal of the study was to evaluate whether an optical fiber-based technique can be used to quantify the expression of multiple growth factor receptors simultaneously. METHODS: The authors examined HER2 expression using the monoclonal antibody trastuzumab as a targeting ligand to test their system. They conjugated trastuzumab to 2 different Alexa Fluor dyes with different excitation and emission wavelengths. Two of the dye conjugates were subsequently injected intravenously into mice bearing HER2-expressing subcutaneous tumors. An optical fiber was then inserted into the tumor through a 30-gauge needle, and using a single laser beam as the excitation source, the fluorescence emitted by the 2 conjugates was identified and quantified by 2-photon optical fiber fluorescence. RESULTS: The 2 conjugates bound to the HER2-expressing tumor competitively in a receptor-specific fashion, but they failed to bind to a similar cell tumor that did not express HER2. The concentration of the conjugate present in the tumor as determined by 2-photon optical fiber fluorescence was shown to serve as an index of the HER2 expression levels.
關聯: Cancer, 118(8): 2148-2156
显示于类别:[電機工程學系] 期刊論文

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