National Changhua University of Education Institutional Repository : Item 987654321/12042

English | 正體中文 | 简体中文 | Items with full text/Total items : 6507/11669
Visitors : 29927395 Online Users : 442

RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Scope Adv. Search

Login ‧ Upload ‧ Help ‧ About ‧ Administer

NCUEIR > College of Science > Department of Chemistry > Periodical Articles > Item 987654321/12042

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12042

Title:	Charge Density Profiling of Circulating Human Low-density Lipoprotein Particles by Capillary Zone Electrophoresis
Authors:	Liu, Mine-Yine;McNeal, Catherine J.;Macfarlane, Ronald D.
Contributors:	化學系
Date:	2004-09
Issue Date:	2012-07-03T03:29:36Z
Publisher:	John Wiley & Sons Ltd.
Abstract:	Capillary zone electrophoresis (CZE) has been utilized to profile the low-density (LDL) particles in human blood serum in this study. A 5 mM sodium phosphate buffer, pH 7.40, was chosen as the most suitable CE buffer and an extensive ultrafiltration (UF) procedure was applied to purify the LDL sample. Two LDL particle species, LDL with lower mobility and LDL− with higher mobility were observed. The electropherograms were highly reproducible with good precision of effective mobilities, corrected peak areas (CPAs) and CPA ratio of LDL−/LDL. LDL particles shown on the electropherogram were also characterized by several procedures. The applications of Sigma HDL cholesterol reagent and CE on-line 2-propanol precipitation indicated that the two particle species shown in the electropherogram belong to LDL. The LDL particles were found to associate with the buoyant LDL fraction and the LDL− particles associate with the dense LDL fraction. This study utilizes CZE for the profiling of LDL isoforms and provides a new analytical method for the resolution of LDL subspecies. It demonstrates a high-mobility LDL particle which circulates in healthy subjects and diminishes in atherosclerotic patients. Diminution of the high-mobility LDL subspecies may be linked to minimal formation of arterial plaque in atherosclerotic patients.
Relation:	Electrophoresis, 25(17): 2985-2995
Appears in Collections:	[Department of Chemistry] Periodical Articles

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	612	View/Open

Loading...