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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12044

Title: In Vitro Regulation of Single Collagen Fibril Length by Buffer Compositions and Temperature
Authors: Liu, Mine-Yine;Yeh, Ming L.;Luo, Zong P.
Contributors: 化學系
Date: 2005-11
Issue Date: 2012-07-03T03:29:40Z
Publisher: IOS Press
Abstract: An understanding of collagen ultrastructure is very important for designing biopolymers mimicking collagen functions in tissue engineering, or for diagnosing abnormal collagen structure in clinical study. The present study examined formation of a large population of type I collagen single fibrils under different buffer compositions and temperatures. Fibril structures were investigated by dark-field microscopy and atomic force microscopy (AFM). In the phosphate buffered saline (PBS) buffer, the average lengths of single fibrils were 4.8±2.2, 5.0±1.9 and 9.2±5.0 μm for 37°C, 33°C and 29°C, respectively. The differences were significant (P<0.05) between 37°C and 29°C and between 33°C and 29°C. In the sodium phosphate (SP) buffer, the average lengths of single fibrils were 10.6±5.4, 11.1±4.5 and 19.6±11.7 μm for 37°C, 33°C and 29°C, respectively. Similarly, the differences were significant (P<0.05) between 37°C and 29°C and between 33°C and 29°C. While at the same temperature, the average lengths of single fibrils differed significantly (P<0.05) between PBS and SP buffers. Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer.
Relation: Bio-Medical Materials and Engineering, 15(6): 413-420
Appears in Collections:[化學系] 期刊論文

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