National Changhua University of Education Institutional Repository : Item 987654321/12046
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题名: Separation and Determination of in Vitro Oxidized Phospholipids by Capillary Zone Electrophoresis
作者: Ho, Yu-Ling;Chiu, Jing-Huei;Wu, Chung-Yu;Liu, Mine-Yine
贡献者: 化學系
关键词: Aqueous-organic buffer system;Capillary zone electrophoresis;Copper sulfate;In vitro oxidation;Phosphatidyl choline
日期: 2007-08
上传时间: 2012-07-03T03:29:44Z
出版者: Academic Press
摘要: A simple capillary zone electrophoresis (CZE) method was used to determine in vitro oxidized phosphatidyl choline (ox-PC). To optimize the capillary electrophoresis (CE) conditions, organic buffer additives, buffer ionic strength, buffer pH, and applied voltage were examined. The optimal CE separation buffer chosen was an aqueous-organic solvent system containing 10% sodium phosphate buffer (5 mM, pH 7.40), 80% methanol, and 10% acetonitrile. One major peak with a small shoulder was found for phosphatidyl choline (PC), whereas one major peak and a complex region containing several lower-mobility peaks were found for ox-PC. The lower-mobility species of ox-PC has high levels of conjugated dienes characterized by strong absorbance at 234 nm. The electropherograms of PC and ox-PC were significantly different and highly reproducible. The intensities of lower-mobility species decreased significantly when the antioxidant vitamin C concentration was increased from 6 to 600 μM. This study provides a simple CZE method to differentiate in vitro oxidized from nonoxidized PC molecular species. © 2007 Elsevier Inc. All rights reserved.
關聯: Analytical Biochemistry, 367(2): 210-218
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