National Changhua University of Education Institutional Repository : Item 987654321/12048
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6507/11669
Visitors : 29723513      Online Users : 379
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12048

Title: In Vitro Oxidized and Glycated Human Low-density Lipoprotein Particles Characterized by Capillary Zone Electrophoresis
Authors: Chiu, Jing-Huei;Peng, Yu-Nong;Yang, Ying-Ling;Tsai, Ming-Hua;Ho, Yu-Ling;Wu, Chung-Yu;Liu, Mine-Yine
Contributors: 化學系
Keywords: Capillary zone electrophoresis;In vitro glycation;In vitro oxidation;Low-density lipoprotein
Date: 2008-11
Issue Date: 2012-07-03T03:29:47Z
Publisher: Elsevier
Abstract: A simple capillary zone electrophoresis (CZE) method was used to determine native, in vitro Cu2+ and glucose modified low-density lipoprotein (LDL) particles for four healthy subjects. The LDL electropherograms are highly reproducible with good precisions of effective mobility and peak area. The native LDL capillary electrophoresis (CE) profile shows a major peak with lower mobility and two minor peaks with higher mobilities. For three-hour Cu2+ oxidation, one major peak with mobility close to that of the native major peak, and one minor peak with mobility extending to -47 � 10-5 cm2 V-1 s-1 appear. For eighteen-hour Cu2+ oxidation, one major peak with mobility much higher than that of the native major peak appears. As the reaction time for LDL and Cu2+ increases from 0 to 24 h, effective mobility of the LDL major peak increases, suggesting that LDL particles become more negatively charged and oxidized as the time increases. The in vitro glycated LDL particles are characterized by a major peak and two minor peaks. Mobility of the major peak is close to that of native major peak, but the second minor peak is much more negatively charged with mobility extending to -53 � 10-5 cm2 V-1 s-1. Native, oxidized and glycated LDL particles show distinctive differences in their CZE profiles. Agarose electrophoresis shows that the charge to mass ratios of native, three-hour Cu2+ and glucose modified LDL particles are similar, but that of eighteen-hour Cu2+ oxidized LDL particles is higher. � 2008 Elsevier B.V. All rights reserved.
Relation: Journal of Chromatography B, 875(2): 383-391
Appears in Collections:[Department of Chemistry] Periodical Articles

Files in This Item:

File SizeFormat
index.html0KbHTML689View/Open


All items in NCUEIR are protected by copyright, with all rights reserved.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback