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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12223

Title: Nitric Oxide Synthase Activity and Immunoreactivity in the Crayfish Procambarus Clarkii
Authors: Lee, Chi-Ying;Zou, Hong-Shin;Yau, Shiou-Mei;Ju, Yuh-Rur;Liau, Ching-Sheyen
Contributors: 生物學系
Keywords: Anti-nitric oxide synthase;Griess reaction;Nitric oxide;Nitric oxide synthase;Neurosecretion;Procambarus clarkii;Vision
Date: 2000-04
Issue Date: 2012-07-03T04:02:26Z
Publisher: Lippincott Williams & Wilkins
Abstract: The presence, characteristics and localization of nitric oxide synthase (NOS) in the eyestalk ganglia of the crayfish (Procambarus clarkii) were investigated. Biochemical assay indicated that NOS activity was significantly inhibited by NG-nitro-L-arginine (a NOS inhibitor), fluphenazine-N-2-chloroethane (a calmodulin inhibitor) as well as by omitting β-NADPH or Ca2+ from the incubations. Immunohistochemical studies localized NOS immunoreactivity (IR) to the first chiasma and the sinus gland. Finally, a NOS-immunoreactive protein (138 kDa) was detected by Western blot analysis. These data demonstrate for the first time the existence in crustacean eyestalk ganglia of NOS with biochemical characteristics similar to mammalian constitutive NOS. The localization of NOS-IR suggests that NO may be involved in regulating visual processes and neuroendocrine function in crustaceans.
Relation: NeuroReport, 11(6): 1273-1276
Appears in Collections:[生物學系] 期刊論文

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