National Changhua University of Education Institutional Repository : Item 987654321/12223
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题名: Nitric Oxide Synthase Activity and Immunoreactivity in the Crayfish Procambarus Clarkii
作者: Lee, Chi-Ying;Zou, Hong-Shin;Yau, Shiou-Mei;Ju, Yuh-Rur;Liau, Ching-Sheyen
贡献者: 生物學系
关键词: Anti-nitric oxide synthase;Griess reaction;Nitric oxide;Nitric oxide synthase;Neurosecretion;Procambarus clarkii;Vision
日期: 2000-04
上传时间: 2012-07-03T04:02:26Z
出版者: Lippincott Williams & Wilkins
摘要: The presence, characteristics and localization of nitric oxide synthase (NOS) in the eyestalk ganglia of the crayfish (Procambarus clarkii) were investigated. Biochemical assay indicated that NOS activity was significantly inhibited by NG-nitro-L-arginine (a NOS inhibitor), fluphenazine-N-2-chloroethane (a calmodulin inhibitor) as well as by omitting β-NADPH or Ca2+ from the incubations. Immunohistochemical studies localized NOS immunoreactivity (IR) to the first chiasma and the sinus gland. Finally, a NOS-immunoreactive protein (138 kDa) was detected by Western blot analysis. These data demonstrate for the first time the existence in crustacean eyestalk ganglia of NOS with biochemical characteristics similar to mammalian constitutive NOS. The localization of NOS-IR suggests that NO may be involved in regulating visual processes and neuroendocrine function in crustaceans.
關聯: NeuroReport, 11(6): 1273-1276
显示于类别:[生物學系] 期刊論文

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