National Changhua University of Education Institutional Repository : Item 987654321/12225

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NCUEIR > College of Science > Department of Biology > NSC Projects > Item 987654321/12225

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12225

Title:	甲殼類壓迫反應模式之建立 (I) Establishing a Crustacean Model of Stress Responses
Authors:	李奇英
Contributors:	生物系
Date:	2000
Issue Date:	2012-07-03T04:02:34Z
Publisher:	行政院國家科學委員會
Abstract:	The present studies investigate the roles of serotonergic neuronal pathways in regulating release og CHH and of serotonin-regulated CHH release in stress responses. Eyestalk ganglia with intact X-organ-sinus gland complex were dissected from the crayfish Procambarus clarkii and incubated under various experimental conditions. Incubation media were then analyzed for the presence of released hyperglycemic factor using an in vivo bioassay. The results show that 5-HT enhanced release of hyperglycemic factor in a dose-dependent manner. Further, activity of the 5-HT-released hyperglycemic factor could be liminated by adsorption of incubation media with anti-CHH serum, but not by pre-imminated or anti-5-HT serum. On the other hand, thermal stress significantly increased blood glucose levels in intact animals, but not in eyestalk-ablated animals. Reserpine, a 5HT-depleting drug, was effective in blocking stress-induced hyperglycemia. These results confirm the hypothesis that 5-HT enhances release of CHH that in turn elicits hyperglycemic responses and suggests that 5-HTergic neurons are involved in stress-induced hyperglycemia.
Relation:	國科會計畫, 計畫編號: NSC89-2311-B018-005; 研究期間: 8908-9007
Appears in Collections:	[Department of Biology] NSC Projects

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