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Title: Demonstration of Nitric Oxide Synthase Activity in Crustacean Hemocytes and Anti-microbial Activity of Hemocyte-derived Nitric Oxide
Authors: Yeh, Feng-Ching;Wu, Su-Hua;Lai, Chi-Yung;Lee, Chi-Ying
Contributors: 生物學系
Keywords: Bactericidal activity;Nitric oxide;Nitric oxide synthase;Hemocyte;Lipopolysaccharide;Crustacean
Date: 2006-05
Issue Date: 2012-07-03T04:02:51Z
Publisher: Elsevier
Abstract: We determined the biochemical characteristics of nitric oxide synthase (NOS) in hemocytes of the crayfish Procambarus clarkii and
investigated the roles of hemocyte-derived NO in host defense. Biochemical analysis indicated the presence of a Ca2+-independent NOS activity,
which was elevated by lipopolysaccharide (LPS) treatment. When bacteria (Staphylococcus aureus) and hemocytes were co-incubated, adhesion
of bacteria to hemocytes was observed. NO donor sodium nitroprusside (SNP) significantly increased the numbers of hemocytes to which bacteria
adhered. Similarly, LPS elicited bacterial adhesion and the LPS-induced adhesion was prevented by NOS inhibitor NG-monomethyl-L-arginine (LNMMA).
Finally, plate count assay demonstrated that addition of LPS to the hemocytes/bacteria co-incubation resulted in a significant decrease in
bacterial colony forming unit (CFU), and that L-NMMA reversed the decreasing effect of LPS on CFU. The combined results demonstrate the
presence of a Ca2+-independent LPS-inducible NOS activity in crayfish hemocytes and suggest that hemocyte-derived NO is involved in
promoting bacterial adhesion to hemocytes and enhancing bactericidal activity of hemocytes.
� 2006 Elsevier Inc. All rights reserved.
Relation: Comparative Biochemistry and Physiology-Part B, 144(1): 11-17
Appears in Collections:[Department of Biology] Periodical Articles

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