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Title: Interactions of Chaperonin with a Weakly Active Anthranilate Synthase from the Aphid Endosymbiont Buchnera Aphidicola
Authors: Huang, Chia-Ying;Lee, Chi-Ying;Wu, Hsiao-Chen;Kuo, Mei-Hwa;Lai, Chi-Yung
Contributors: 生物學系
Date: 2008-11
Issue Date: 2012-07-03T04:02:55Z
Publisher: SpringLink
Abstract: The endosymbiotic bacterium Buchnera provides
its aphid host with essential amino acids. Buchnera is typical
of intracellular symbiotic and parasitic microorganisms in
having a small effective population size, which is believed to
accelerate genetic drift and reduce the stability of gene
products. It is hypothesized that Buchnera mitigates protein
instability with an increased production of the chaperonins
GroESL. In this paper, we report the expression and
functional analysis of trpE, a plasmid-borne fast-evolving
gene encoding the tryptophan biosynthesis enzyme anthranilate
synthase. We overcame the problem of low enzyme
stability by using an anthranilate synthase-deficient mutant
of E. coli as the expression host and the method of genetic
complementation for detection of the enzyme activity. We
showed that the Buchnera anthranilate synthase was only
weakly active at the temperature of 26°C but became
inactive at the higher temperatures of 32°C and 37°C and
that the coexpression with chaperonin genes groESL of
E. coli enhanced the function of the Buchnera enzyme.
These findings are consistent with the proposed role of
groESL in the Buchnera–aphid symbiosis.
Relation: Microbial Ecology, 56(4): 696-703
Appears in Collections:[Department of Biology] Periodical Articles

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