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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12248

Title: 甲殼類升血糖激素家族分子與其受體之研究
Studies on CHH Family Peptides and Their Receptors
Authors: 李奇英
Contributors: 生物系
Keywords: Receptor guanylyl cyclase;Crustacean hyperglycemic hormone;Molt-inhibiting hormone;cGMP;Crustacean
鳥苷酸環化酶受體;甲殼類升血糖荷爾蒙;蛻殼抑制荷爾蒙;環鳥苷酸;甲殼類動物
Date: 2005
Issue Date: 2012-07-03T04:03:44Z
Publisher: 行政院國家科學委員會
Abstract: In studies reported here, we used a PCR-based cloning strategy (RT-PCR followed by
5'- and 3'-RACE) to clone from Y-organs a cDNA (CsGC-YO1) encoding a putative rGC.
DNA sequence analysis revealed a 3807 base pair open reading frame encoding a 56 residue
signal peptide and a 1213 residue rGC. Analysis of the deduced amino acid sequence showed
that CsGC-YO1 contains the signature domains characteristic of rGCs, including an
extracellular ligand-binding domain, a single transmembrane domain, a kinase-like domain, a
dimerization domain, and a cyclase catalytic domain. CsGC-YO1 is most closely related to an
rGC from the crayfish, Procambarus claikii (PcGC-M2, 58.4% identity), and rGCs from three
insect species (33.1 - 37.5% identity). Conserved cysteine residues are similarly distributed in
the extracellular domains of CsGC-YO1, PcGC-M2, and the three insect rGCs. RT-PCR
revealed the CsGC-YO1 transcript is strongly expressed in Y-organs and several other tissues.
現有的證據顯示甲殼類升血糖激素 (CHH) 活化細胞膜上之鳥甘酸環化脢,產生 cGMP
(cyclic guanosine 3’,5’-monophosphate) 為二級傳訊者,來傳達 CHH 對於碳水化合物代謝之訊
息。本計畫之成果自 Y-器官選殖一膜型鳥甘酸環化脢基因(PcGC-M2)。分析其推估之胺基酸序
列顯示具有膜型鳥甘酸環化脢所有特有之區域,包含胞外配體結合區、穿膜區、以及胞內激脢同
源區與環化脢催化區、及一段 274 殘基之 C-端段落。PcGC-M2 和螯蝦 (P. clarkii)、果蠅之膜
型鳥甘酸環化脢最為相似;此三種膜型鳥甘酸環化脢其胞外配體結合區有相似之保守性cysteine
殘基之分佈。CsGC-YO1 在數個組織均有表現。
Relation: 國科會計畫, 計畫編號: NSC94-2311-B018-003; 研究期間: 9408-9507
Appears in Collections:[生物學系] 國科會計畫

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