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題名: | 甲殼類升血糖激素家族分子與其受體之研究 Studies on CHH Family Peptides and Their Receptors |
作者: | 李奇英 |
貢獻者: | 生物系 |
關鍵詞: | Receptor guanylyl cyclase;Crustacean hyperglycemic hormone;Molt-inhibiting hormone;cGMP;Crustacean 鳥苷酸環化酶受體;甲殼類升血糖荷爾蒙;蛻殼抑制荷爾蒙;環鳥苷酸;甲殼類動物 |
日期: | 2005
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上傳時間: | 2012-07-03T04:03:44Z
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出版者: | 行政院國家科學委員會 |
摘要: | In studies reported here, we used a PCR-based cloning strategy (RT-PCR followed by 5'- and 3'-RACE) to clone from Y-organs a cDNA (CsGC-YO1) encoding a putative rGC. DNA sequence analysis revealed a 3807 base pair open reading frame encoding a 56 residue signal peptide and a 1213 residue rGC. Analysis of the deduced amino acid sequence showed that CsGC-YO1 contains the signature domains characteristic of rGCs, including an extracellular ligand-binding domain, a single transmembrane domain, a kinase-like domain, a dimerization domain, and a cyclase catalytic domain. CsGC-YO1 is most closely related to an rGC from the crayfish, Procambarus claikii (PcGC-M2, 58.4% identity), and rGCs from three insect species (33.1 - 37.5% identity). Conserved cysteine residues are similarly distributed in the extracellular domains of CsGC-YO1, PcGC-M2, and the three insect rGCs. RT-PCR revealed the CsGC-YO1 transcript is strongly expressed in Y-organs and several other tissues. 現有的證據顯示甲殼類升血糖激素 (CHH) 活化細胞膜上之鳥甘酸環化脢,產生 cGMP (cyclic guanosine 3’,5’-monophosphate) 為二級傳訊者,來傳達 CHH 對於碳水化合物代謝之訊 息。本計畫之成果自 Y-器官選殖一膜型鳥甘酸環化脢基因(PcGC-M2)。分析其推估之胺基酸序 列顯示具有膜型鳥甘酸環化脢所有特有之區域,包含胞外配體結合區、穿膜區、以及胞內激脢同 源區與環化脢催化區、及一段 274 殘基之 C-端段落。PcGC-M2 和螯蝦 (P. clarkii)、果蠅之膜 型鳥甘酸環化脢最為相似;此三種膜型鳥甘酸環化脢其胞外配體結合區有相似之保守性cysteine 殘基之分佈。CsGC-YO1 在數個組織均有表現。 |
關聯: | 國科會計畫, 計畫編號: NSC94-2311-B018-003; 研究期間: 9408-9507 |
顯示於類別: | [生物學系] 國科會計畫
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