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Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/12249

Title: 甲殼類升血糖荷爾蒙受體—鳥甘酸環化之表現與活性分析(2/3)
Crustacean Hyperglycemic Hormone Receptor---Expression and Functional Studies of a Guanylyl Cyclase (II)
Authors: 李奇英
Contributors: 生物系
Keywords: receptor guanylyl cyclase;crustacean hyperglycemic hormone;molt-inhibiting hormone;Cgmp;X-organ-sinus gland;Crustacean
Date: 2005
Issue Date: 2012-07-03T04:03:56Z
Publisher: 行政院國家科學委員會
Abstract: Available data indicate that crustacean hyperglycemic hormone (CHH) stimulates membrane-bound
guanylyl cyclase (GC) producing cyclic guanosine 3’,5’-monophosphate, which in turn mediates the
effect of CHH on carbohydrate metabolism. In the first year of the project, we achieved the cloning of a
cDNA encoding a GC-like protein (PcGC-M2) from muscle of the crayfish, Procambarus clarkii. The
cDNA (PcGC-M2) encodes a protein with the signature domains characteristic of membrane form GCs,
including an extracellular ligand-binding domain, a single transmembrane, and intracellular kinase-like
and cyclase catalytic domains. The tissue distribution of the PcGC-M2 transcript is consistent with the
hypothesis that it encodes a CHH receptor. Now, in the second year of the project, we reported the
cloning of a cDNA encoding a truncated GC-like protein (PcGC-M2’), which possesses only the
extracellular domain, and transmembrane domain, and a partial kinase homology domain, but lacks a
cyclase catalytic domain. Furthermore, antibodies against the extracellular domain of PcGC-M2 were
raised, purified, and tested for immunoreactivity. Heterologous expression of PcGC-M2, PcGC-M2’
was carried out in insect S2 cells or HEK293 cells. Optimal protocol for heterologous expression is
being tested. The expressed proteins will provide a foundation for future studies of the signaling
cascade initiated by CHH.
Relation: 國科會計畫, 計畫編號: NSC94-2311-B018-001; 研究期間: 9408-9507
Appears in Collections:[生物學系] 國科會計畫

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