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題名: | 甲殼類升血糖荷爾蒙受體—鳥甘酸環化之表現與活性分析(2/3) Crustacean Hyperglycemic Hormone Receptor---Expression and Functional Studies of a Guanylyl Cyclase (II) |
作者: | 李奇英 |
貢獻者: | 生物系 |
關鍵詞: | receptor guanylyl cyclase;crustacean hyperglycemic hormone;molt-inhibiting hormone;Cgmp;X-organ-sinus gland;Crustacean 鳥苷酸環化酶受體;甲殼類升血糖荷爾蒙;蛻殼抑制荷爾蒙;環鳥苷酸;X-器官/血竇腺;甲殼類動物 |
日期: | 2005
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上傳時間: | 2012-07-03T04:03:56Z
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出版者: | 行政院國家科學委員會 |
摘要: | Available data indicate that crustacean hyperglycemic hormone (CHH) stimulates membrane-bound guanylyl cyclase (GC) producing cyclic guanosine 3’,5’-monophosphate, which in turn mediates the effect of CHH on carbohydrate metabolism. In the first year of the project, we achieved the cloning of a cDNA encoding a GC-like protein (PcGC-M2) from muscle of the crayfish, Procambarus clarkii. The cDNA (PcGC-M2) encodes a protein with the signature domains characteristic of membrane form GCs, including an extracellular ligand-binding domain, a single transmembrane, and intracellular kinase-like and cyclase catalytic domains. The tissue distribution of the PcGC-M2 transcript is consistent with the hypothesis that it encodes a CHH receptor. Now, in the second year of the project, we reported the cloning of a cDNA encoding a truncated GC-like protein (PcGC-M2’), which possesses only the extracellular domain, and transmembrane domain, and a partial kinase homology domain, but lacks a cyclase catalytic domain. Furthermore, antibodies against the extracellular domain of PcGC-M2 were raised, purified, and tested for immunoreactivity. Heterologous expression of PcGC-M2, PcGC-M2’ was carried out in insect S2 cells or HEK293 cells. Optimal protocol for heterologous expression is being tested. The expressed proteins will provide a foundation for future studies of the signaling cascade initiated by CHH. |
關聯: | 國科會計畫, 計畫編號: NSC94-2311-B018-001; 研究期間: 9408-9507 |
顯示於類別: | [生物學系] 國科會計畫
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