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NCUEIR > College of Science > biotech > Periodical Articles >  Item 987654321/13210

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/13210

Title: Inhibitory Effects of Du-zhong (Eucommia ulmoides Oliv.) Against Low-density Lipoprotein Oxidative Modification
Authors: Yen, Gow-Chin;Hsieh, Chiu-Lan
Contributors: 生物技術研究所
Keywords: Eucommia ulmoides Oliv.;Low-density lipoprotein;Protocatechuic acid
Date: 2002-06
Issue Date: 2012-08-07T06:56:40Z
Publisher: Elsevier
Abstract: This study presents the effects of water extracts from leaves, raw cortex, and roasted cortex of Du-zhong (Eucommia ulmoides Oliv.) on the oxidative modification of human low-density lipoprotein (LDL) induced by copper ion (Cu2+). Through determination of the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes (CD), as well as relative electrophoretic mobility (REM), all extracts of Du-zhong were found to possess inhibitory effects on the oxidative modification of LDL induced by Cu2+. The leaf extract had the most significant inhibitory effect. The ethyl acetate extract from leaf extract of Du-zhong was separated into six bands by preparative TLC. Band 2 (Rf=0.40), which was most inhibitory against LDL oxidative modification, was analyzed by HPLC; a single, main peak was collected and further examined using NMR and MS. The antioxidant compound thus identified was protocatechuic acid (PCA). At a concentration of 10 μg/ml, PCA showed a stronger inhibitory effect against LDL oxidative modification induced by Cu2+ than did Du-zhong extracts or ascorbic acid at the same concentration. The results suggest that, in leaf extract of Du-zhong, PCA could be a major source of the inhibitory effect against LDL oxidative modification induced by Cu2+.
Relation: Food Chemistry, 77(4): 449-456
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