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Title: Inhibitory Effect of Eucommia ulmoides Oliv. on Oxidative DNA Damage in Lymphocytes Induced by H2O2
Authors: Yen, Gow-Chin;Hsieh, Chiu-Lan
Contributors: 生物技術研究所
Keywords: Eucommia ulmoides;DNA damage;Comet;Lymphocyte;H2O2
Date: 2003
Issue Date: 2012-08-07T06:56:42Z
Publisher: John Wiley & Sons, Inc.
Abstract: This study used the alkaline single cell gel electrophoresis assay (comet assay) to
investigate the effect of water extracts of roasted cortex and leaves from Duzhong
on DNA damage in lymphocytes induced by H2O2. The results showed
that the DNA damage in human lymphocytes increased with an increase in the
concentration of H2O2 (0–200 mM), but that the water extracts from Du-zhong
(0–2 g l 1) only slightly affected DNA damage. The inhibitory effect of leaf
extract on DNA damage induced by H2O2 in lymphocytes was more significant
(Po0.05) than that of roasted cortex. Leaf extract showed a rather significant
inhibitory effect in a concentration-dependent manner. At a concentration of
2 g l 1, the leaf extract inhibited 37.9% DNA oxidative damage in human
lymphocytes. In order to elucidate the mechanism of the leaf extract suppression
effect on DNA damage induced by H2O2 in lymphocytes, an experiment was
divided with six groups (A–F). Group A was used to evaluate the repair ability of
the leaf extract for DNA damage; Group B was employed to determine the
scavenging ability on H2O2; and Group C was studied to assess the ability of leaf
extract to increase the defense capability. Groups D–F were negative controls and
blank. The results showed that group B had the best inhibitory effect. Also, leaf
extract had significant ability to scavenge H2O2 in an in vitro HRP-phenol red
test. Thus, it appears that H2O2 scavenging potency may be the major mechanism
whereby leaf extract inhibits oxidative DNA damage induced by H2O2.
Teratogenesis Carcinog.
Relation: Teratogenesis, Carcinogenesis, and Mutagenesis Supplement, 1: 23-34
Appears in Collections:[生物技術研究所] 期刊論文

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