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http://ir.ncue.edu.tw/ir/handle/987654321/13216
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| Title: | Brain Derived Metastatic Prostate Cancer DU-145 Cells are Effectively Inhibited in Vitro by Guava (Psidium gujava L.) Leaf Extracts |
| Authors: | Chen, Kuan-Chou;Hsieh, Chiu-Lan;Peng, Chiung-Chi;Hsieh-Li, Hsiu-Mei;Chiang, Han-Sun;Huang, Kuan-Dar;Peng, Robert Y. |
| Contributors: | 生物技術研究所 |
| Date: | 2007
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| Issue Date: | 2012-08-07T06:56:53Z
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| Publisher: | Taylor&Francis |
| Abstract: | The aqueous extract of Psidium guajava L. (PE) inhibited the cancer cell DU-145 in a dose- and time-dependent manner. At 1.0 mg/mL, PE reduced the viability of PCa DU-145 (the androgen independent PCa cells) to 36.1 and 3.59%, respectively after 48 h and 72 h of incubations. The absolute cell viability suppressing capability (VSC)AC could reach 262.5 cells-mL-h/mg on exposure to PE for 72 h, corresponding to the safe ranges, i.e. the percent viability suppressing rates (PVSR) of 2.72 and 2.41 folds for DU-145 comparing to PZ-HPV-7 cells when treated with PE at 0.5 and 1.0 mg/mL respectively for 72 h. In addition, the colony forming capability of DU-145 cells was apparently lowered. The suppressing rates of which reached 8.09 and 5.96 colony/mg/day for D-145 and PZ-HPV-7 cells, respectively within the concentration range of PE at 0.1 0.25 mg/mL. Cell cycle arrests at G0/G1 phase in both cells were observed by TUNEL assay and flow cytometric analysis, yet more prominently evident in DU-145. In addition, suppression of the matrix metalloproteinases MMP-2 and MMP-9, and the upregulation of active caspase-3 at 0.10 to 1.0 mg/mL in DU-145 were also effected in a dose-dependent manner by PE at 0.25 to 1.0 mg/mL, implicating a potent anti-metastasis power of PE. Conclusively, we ascribe the anticancer activity of PE to its extraordinarily high polyphenolic (165.61 � 10.39 mg/g) and flavonoid (82.85 � 0.22 mg/g) contents. Furthermore, PE might be useful for treatment of brain derived metastatic cancers such as DU-145, acting simultaneously as both a chemopreventive and a chemotherapeutic. |
| Relation: | Nutrition and Cancer, 58(1): 93-106 |
| Appears in Collections: | [生物技術研究所] 期刊論文
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