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Title: Anticancer Activity of Rhamnoallosan Against DU-145 Cells Is Kinetically Complementary to Coexisting Polyphenolics in Psidium Guajava Budding Leaves
Authors: Chen, Kuan-Chou;Hsieh, Chiu-Lan;Huang, Kuan-Dar;Ker, Yaw-Bee;Chyau, Charng-Cherng;Peng, Robert Y.
Contributors: 生物技術研究所
Keywords: Rhamnoallosan peptidoglycans;Rhamnose;Allose;Prostate cancer;Psidium guajava budding leaves
Date: 2009-07
Issue Date: 2012-08-07T06:57:58Z
Publisher: American Chemical Society
Abstract: Psidium guajava L. is a valuable farm fruit plant having many medicinal uses. Previously its budding leaves (PE) were shown to contain huge amounts of soluble polyphenolics (SP) including (in mg/g) gallic acid (348), catechin (102), epicatechin (60), rutin (100), quercetin (102), and rutin (100) and to exhibit potent anticancer activity. However, reconstitution of these polyphenolics recovered only 40% of the original bioactivity, and the soluble carbohydrate (SC) portion in PE was suspected to contribute the remaining. PE contained a novel rhamnoallosan, which had a carbohydrate/protein (w/w) ratio = 29.06%/10.27% (=2.83, average molecular mass of 5029 kDa), characteristically evidencing a peptidoglycan, consisting of a composition (mole % ratio) of rhamnose/allose/arabinose/tallose/xylose/fucose/glucose/mannose/galactose = 36.05:24.24:8.76:7.95:7.37:5.90:3.69:3.19:2.85 and of amino acid (in wt %) glycine/leucine/proline/alanine/methionine/isoleucine/valine/histidine/tyrosine/phenylalanine/cysteine/aspartic acid/lysine/glutamic acid = 37.12:12.68:10.05:8.97:5.99:4.89:4.83:4.25:4.05:2.78:1.86:1.10:0.73:0.70. Kinetic analysis showed comparable apparent cell-killing rate coefficients (k(app)) to be 4.03 x 10(3) and 2.92 x 10(3) cells mg(-1) h(-1), respectively, by SP and SC, evidencing the complementary anti-DU-145 bioactivity in nature.
Relation: Journal of Agricultural and Food Chemistry, 57(14): 6114-6122
Appears in Collections:[生物技術研究所] 期刊論文

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