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题名: Phase- and Age-Related Differences in Protein Tyrosine Phosphorylation in Sequoia Sempervirens
作者: Huang, L. C.;Pu, S. Y.;Murashige, T.;Fu, Shin-Feng;Kuo, T. T.;Huang, D. D.;Huang, H. J.
贡献者: 生物學系
关键词: Aging;Rejuvenation;Signal transduction
日期: 2004
上传时间: 2013-01-07T02:28:42Z
出版者: SpringerLink
摘要: Apical and basal halves of 3 cm long apical segments of in vitro cultured juvenile, adult and rejuvenated Sequoia
sempervirens shoots were analyzed for total and tyrosine phosphorylated proteins. The latter was detected by a
phosphotyrosine specific antibody. Younger tissues, or the apical halves of shoot terminals, showed larger amounts of
36, 44, 46 kDa proteins and lesser amounts of 29 kDa proteins. These are proposed as age-related changes. Phase-related
proteins were also evident. Adult tissues contained more of the 34 and 36 kDa proteins than juvenile and rejuvenated
shoots. Western blotting with a phosphotyrosine specific antibody revealed more of 25, 39, and 54 kDa protein in the
younger tissues. In addition, tyrosine phosphorylated proteins of 25 and 34 kDa were higher in the adult, than in juvenile
or rejuvenated tissues. Our findings showed that protein tyrosine phosphorylation, or the signal transduction pathway, is
involved in phase- and age-related processes.
關聯: Biologia Plantarum, 47(4): 601-603
显示于类别:[生物學系] 期刊論文


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