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Title: Genetic Modification of Alternative Respiration in Nicotiana Benthamiana Affects Basal and Salicylic Acid-Induced Resistance to Potato Virus X
Authors: Lee, Wing-Sham;Fu, Shih-Feng;Jeanmarie Verchot-Lubicz;Carr, John P.
Contributors: 生物學系
Date: 2011
Issue Date: 2013-01-07T02:28:42Z
Publisher: BioMed Central Ltd.
Abstract: Background: Salicylic acid (SA) regulates multiple anti-viral mechanisms, including mechanism(s) that may be
negatively regulated by the mitochondrial enzyme, alternative oxidase (AOX), the sole component of the
alternative respiratory pathway. However, studies of this mechanism can be confounded by SA-mediated induction
of RNA-dependent RNA polymerase 1, a component of the antiviral RNA silencing pathway. We made transgenic
Nicotiana benthamiana plants in which alternative respiratory pathway capacity was either increased by constitutive
expression of AOX, or decreased by expression of a dominant-negative mutant protein (AOX-E). N. benthamiana
was used because it is a natural mutant that does not express a functional RNA-dependent RNA polymerase 1.
Results: Antimycin A (an alternative respiratory pathway inducer and also an inducer of resistance to viruses) and
SA triggered resistance to tobacco mosaic virus (TMV). Resistance to TMV induced by antimycin A, but not by SA,
was inhibited in Aox transgenic plants while SA-induced resistance to this virus appeared to be stronger in Aox-E
transgenic plants. These effects, which were limited to directly inoculated leaves, were not affected by the
presence or absence of a transgene constitutively expressing a functional RNA-dependent RNA polymerase
(MtRDR1). Unexpectedly, Aox-transgenic plants infected with potato virus X (PVX) showed markedly increased
susceptibility to systemic disease induction and virus accumulation in inoculated and systemically infected leaves.
SA-induced resistance to PVX was compromised in Aox-transgenic plants but plants expressing AOX-E exhibited
enhanced SA-induced resistance to this virus.
Conclusions: We conclude that AOX-regulated mechanisms not only play a role in SA-induced resistance but also
make an important contribution to basal resistance against certain viruses such as PVX.
Relation: BMC Plant Biology, 11: 41
Appears in Collections:[Department of Biology] Periodical Articles

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