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Please use this identifier to cite or link to this item:
http://ir.ncue.edu.tw/ir/handle/987654321/15199
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| Title: | Efficient Methods for Isolating Mitochondrial DNA from Fresh or Fixed Molluscan Specimens
從不同性質之軟體動物標本抽取粒線體DNA之方法 |
| Authors: | Jiang, Ling;Wu, W. L.;Lin, Y. S. |
| Contributors: | 生物學系 |
| Keywords: | Mollusks;MtDNA;Isolation
軟體動物;抽取方法 |
| Date: | 1997-01
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| Issue Date: | 2013-01-07T09:08:09Z
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| Publisher: | 中央研究院動物研究所 |
| Abstract: | Fast and efficient methods of isolating mtDNA from molluscan specimens with different conditions have been developed. The standard method involves isolation and purification of a large amount of mtDNA to moderate purity from regular samples, even these containing jelly, mucus, or lipids; the 2nd method extracts mtDNA from a small quantity of sample, such as from 1 trochophore; and the 3rd method obtains mtDNA from formalin-or alcohol-fixed specimens.
本文針對性質不同的動物標本，提供三種快速而有效的mtDNA抽取方法，期望經由這些技術的建立，使得mtDNA相關的研究工作，被更加廣泛地運用。第一個方法，適用於一般較大量的動物標本，即使含有膠質、黏液、或脂肪，亦可得到大量、純淨的mtDNA。第二個方法，能夠從像一個擔輪子幼蟲那麼少量的標本中抽取出mtDNA。第三個方法，可從長期被酒精、或福馬林固定的標本中，僅需三個小時就可抽取出mtDNA。 |
| Relation: | Zoological Studies, 36(1): 74-78 |
| Appears in Collections: | [生物學系] 期刊論文
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| 2020300810002.pdf | 47Kb | Adobe PDF | 379 | View/Open |
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