English  |  正體中文  |  简体中文  |  Items with full text/Total items : 6491/11663
Visitors : 24505553      Online Users : 88
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/15199

Title: Efficient Methods for Isolating Mitochondrial DNA from Fresh or Fixed Molluscan Specimens
從不同性質之軟體動物標本抽取粒線體DNA之方法
Authors: Jiang, Ling;Wu, W. L.;Lin, Y. S.
Contributors: 生物學系
Keywords: Mollusks;MtDNA;Isolation
軟體動物;抽取方法
Date: 1997-01
Issue Date: 2013-01-07T09:08:09Z
Publisher: 中央研究院動物研究所
Abstract: Fast and efficient methods of isolating mtDNA from molluscan specimens with different conditions have been developed. The standard method involves isolation and purification of a large amount of mtDNA to moderate purity from regular samples, even these containing jelly, mucus, or lipids; the 2nd method extracts mtDNA from a small quantity of sample, such as from 1 trochophore; and the 3rd method obtains mtDNA from formalin-or alcohol-fixed specimens.
本文針對性質不同的動物標本,提供三種快速而有效的mtDNA抽取方法,期望經由這些技術的建立,使得mtDNA相關的研究工作,被更加廣泛地運用。第一個方法,適用於一般較大量的動物標本,即使含有膠質、黏液、或脂肪,亦可得到大量、純淨的mtDNA。第二個方法,能夠從像一個擔輪子幼蟲那麼少量的標本中抽取出mtDNA。第三個方法,可從長期被酒精、或福馬林固定的標本中,僅需三個小時就可抽取出mtDNA。
Relation: Zoological Studies, 36(1): 74-78
Appears in Collections:[生物學系] 期刊論文

Files in This Item:

File SizeFormat
2020300810002.pdf47KbAdobe PDF379View/Open


All items in NCUEIR are protected by copyright, with all rights reserved.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback