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NCUEIR > College of Science > Department of Biology > Periodical Articles > Item 987654321/15199

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/15199

Title:	Efficient Methods for Isolating Mitochondrial DNA from Fresh or Fixed Molluscan Specimens 從不同性質之軟體動物標本抽取粒線體DNA之方法
Authors:	Jiang, Ling;Wu, W. L.;Lin, Y. S.
Contributors:	生物學系
Keywords:	Mollusks;MtDNA;Isolation 軟體動物;抽取方法
Date:	1997-01
Issue Date:	2013-01-07T09:08:09Z
Publisher:	中央研究院動物研究所
Abstract:	Fast and efficient methods of isolating mtDNA from molluscan specimens with different conditions have been developed. The standard method involves isolation and purification of a large amount of mtDNA to moderate purity from regular samples, even these containing jelly, mucus, or lipids; the 2nd method extracts mtDNA from a small quantity of sample, such as from 1 trochophore; and the 3rd method obtains mtDNA from formalin-or alcohol-fixed specimens. 本文針對性質不同的動物標本，提供三種快速而有效的mtDNA抽取方法，期望經由這些技術的建立，使得mtDNA相關的研究工作，被更加廣泛地運用。第一個方法，適用於一般較大量的動物標本，即使含有膠質、黏液、或脂肪，亦可得到大量、純淨的mtDNA。第二個方法，能夠從像一個擔輪子幼蟲那麼少量的標本中抽取出mtDNA。第三個方法，可從長期被酒精、或福馬林固定的標本中，僅需三個小時就可抽取出mtDNA。
Relation:	Zoological Studies, 36(1): 74-78
Appears in Collections:	[Department of Biology] Periodical Articles

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