National Changhua University of Education Institutional Repository : Item 987654321/15202

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NCUEIR > College of Science > Department of Biology > NSC Projects > Item 987654321/15202

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/15202

Title:	The Study of Evolution and Taxonomy in Bivalvia Based on mtDNA Analysis 應用mtDNA探討雙殼綱動物的演化和分類
Authors:	Jiang, Ling
Contributors:	生物學系
Keywords:	Bivalvia;Veneridae;Phylogeny;Classification 雙殼綱;簾蛤科;系統發育;分類
Date:	2000
Issue Date:	2013-01-07T09:08:49Z
Publisher:	行政院國家科學委員會
Abstract:	The phylogenetic relationship based on partial sequences of the cytochrome coxidase subunit I gene and the 16S RNA gene in mtDNA to reconstuct the systematics of the Veneridae Family in Bivalvia. Nucleotide sequences of 21 species of venerid clams were used in this study, including 11 examined species and 2 outgroup species colleted from Taiwan, 8 speciesfrom Genebank. About 550bp of 16S RNA gene and 650 bases of COI gene were sequenced to obtain 33 haplotypes in each gene. Phylogenetic trees were established by neighbor-joining(NJ), maximum parsimony(MP) methods and their bootstrap test using Corbucula s., Nucula sp. and Mytilus sp. as the outgroup. From genetic distances and all phylogenetic trees pointed that venerid clams perhaps not be the mpnophyletic groups for Corbucula sp. and Nucula sp. not separating from other taxa of Veneridae. A surprised resuls of Tapes literatus, Tapes philippinarum, Rudittapes variegatus, and Rudittapes philippinarum, these 4 species with similar valve morphs, were suggested to be only one species. The specimens of different sizes in Meretrix lusoria species supposed to be the same species. The specimens of different sizes in Cyclina sinensis species supposed to belong the two different species. 依據粒線體之16S RNA基因和COI基因部分序列的系統發生關係重建雙殼綱簾蛤科(Veneridea)分類系統。共採21簾蛤科動物進行分析，其中11種和兩種外群動物(Corbuchla sp.和Nucula sp)採自台灣，其餘8種的基因序列來自GCG的基因庫。實驗得到約550bp之16S RNA基因和約650bp之COI基因部分序列，二者均有33個基因型。系統發生樹以neighbor-joining(NJ), maximum parsimony(MP) methods和bootstrap test檢測可信度，結果發現連蛤科動物並非單係群，因為蜆科動物在系統樹中無法與其分離。Tapes literatus, Tapes philippinarum, Rudittapes variegatus, and Rudittapes philippinarum四種外型相近的種，從遺傳距離、基因形式和系統樹結果發現應為同一個種。大型文蛤和小型文蛤雖然外型大小有明顯區別，但在遺傳距離、基因形式和系統樹結果中顯示，其乃為同一種。而大型環蚊蛤和小型環蚊蛤不僅外型大小有明顯區別，其遺傳距離、基因形式和系統樹分析結果，均顯示其為兩個不同的種，且親緣關係分長的遠。
Relation:	國科會計畫, 計畫編號: NSC89-2311-B018-003; 研究期間: 8808-8912
Appears in Collections:	[Department of Biology] NSC Projects

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