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|Title: ||DNA analysis on microfabricated electrophoretic devices with bubble cells|
|Authors: ||Tseng, W.-L.;Lin, Y.-W.;Chen, K.-C.;Chang, H.-T.|
|Keywords: ||Bubble cell|
|Issue Date: ||2010-11-10T06:23:55Z
|Abstract: ||Microfluidic devices with bubble cells have been fabricated on poly(methyl methacrylate) (PMMA) plates and have been employed for the analysis of DNA using polyethylene oxide (PEO) solutions. First, the separation channel was fabricated using a wireimprinting method. Then, wires with greater sizes or a razor blade glued in a polycarbonate
plate was used to fabricate bubble cells, with sizes of 190–650 m. The
improvements in resolution and sensitivity have been achieved for large DNA ( 603 base pair, bp) using such devices, which depend on the geometry of the bubble cell. The main contributor for optimal resolution is mainly due to DNA migration at lower electric field strengths inside the bubble cell. On the other hand, slight losses of resolution for small DNA fragments have been found mainly due to diffusion, supported by the loss of resolution when separating two small solutes. With a bubble cell of 75 m (width) 500 m (depth), the sensitivity improvement up to 17-fold has been achieved for the 271 bp fragment in the separation of X-174/HaeIII DNA restriction fragments. We have also found that a microfluidic device with a bubble cell of 360 m 360 m is appropriate for DNA analysis. Such a device has been used for separating DNA ranging
from 8 to 2176 bp and polymerase chain reaction (PCR) products amplified after 30 cycles, with rapidity and improvements in the sensitivity as well as resolution.
|Relation: ||Electrophoresis, 23, 2477-2484|
|Appears in Collections:||[化學系] 期刊論文|
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