National Changhua University of Education Institutional Repository : Item 987654321/1533

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NCUEIR > College of Science > Department of Chemistry > Periodical Articles > Item 987654321/1533

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/1533

Title:	Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis
Authors:	Lin, Y.-W.;Chiu, T.-C.;Chang, H.-T.
Contributors:	化學系
Date:	2003
Issue Date:	2010-11-10T06:28:40Z
Abstract:	Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.
Relation:	J. Chromatogr. B, 793, 37-48
Appears in Collections:	[Department of Chemistry] Periodical Articles

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