National Changhua University of Education Institutional Repository : Item 987654321/1533
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题名: Laser-induced fluorescence technique for DNA and proteins separated by capillary electrophoresis
作者: Lin, Y.-W.;Chiu, T.-C.;Chang, H.-T.
贡献者: 化學系
日期: 2003
上传时间: 2010-11-10T06:28:40Z
摘要: Recent developments in capillary electrophoresis (CE) in conjunction with laser-induced fluorescence (LIF) using long-wavelength (maximum excitation wavelength>500 nm) dyes are reviewed. These dyes are particularly of interest when conducting the analyses of biopolymers by CE-LIF using He-Ne lasers. These systems are benefited from low background, low costs, easy maintenance, and compactness. Derivatizations of DNA and proteins with fluorescent or nonfluorescent chemicals can be carried out prior to, during, or after separations. With the advantages of sensitivity, rapidity, and high efficiency, the applications of CE-LIF to the analysis of polymerase chain reaction products, DNA sequencing, trace analysis of proteins, and single cell analysis have been presented.
關聯: J. Chromatogr. B, 793, 37-48
显示于类别:[化學系] 期刊論文

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