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http://ir.ncue.edu.tw/ir/handle/987654321/1534
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| Title: | Analysis of double-stranded DNA by microchip capillary electrophoresis using polymer solutions containing gold nanoparticles |
| Authors: | Lin, Y.-W.;Huang, M.-J.;Chang, H.-T. |
| Contributors: | 化學系 |
| Date: | 2003
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| Issue Date: | 2010-11-10T06:29:46Z
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| Abstract: | The impact of gold nanoparticles (GNPs) on the microchip electrophoretic separation of double-stranded (ds) DNA using poly(ethylene oxide) (PEO) is described. Coating of the 75-microm separation channel on a poly(methyl methacrylate) (PMMA) plate in sequence with poly(vinyl pyrrolidone), PEO, and 13-nm GNPs is effective to improve reproducibility and resolution. In this study, we have also found that adding 13-nm GNPs to 1.5% PEO is extremely important to achieve high resolution and reproducibility for DNA separation. In terms of the stability of the GNPs, 100 mM glycine-citrate buffer at pH 9.2 is a good buffer system for preparing 1.5% PEO. The separation of DNA markers V and VI ranging in size from 8 to 2176 base pairs has been demonstrated using the three-layer-coated PMMA microdevice filled with 1.5% PEO containing the GNPs. Using these conditions, the analysis of the polymerase chain reaction products of UGT1A7 was complete in 7 min, with the relative standard deviation values of the peak heights and migration times less than 2.3% and 2.0%, respectively. In conjunction with stepwise changes of the concentrations of ethidium bromide (0.5 and 5 microg/ml), this method allows improved resolution and sensitivity for DNA markers V and VI. |
| Relation: | J. Chromatogr. A, 1014, 47-55 |
| Appears in Collections: | [化學系] 期刊論文
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