National Changhua University of Education Institutional Repository : Item 987654321/1539
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题名: Modification of poly(methyl methacrylate) microchannels for highly efficient and reproducible electrophoretic separations of double-stranded DNA
作者: Lin, Y.-W.;Chang, H.-T.
贡献者: 化學系
关键词: DNA separation
Dynamic coating
Gold nanoparticles
Microfluidic devices
Poly(ethylene oxide)
Poly(methyl methacrylate)
日期: 2005
上传时间: 2010-11-10T06:34:36Z
摘要: This paper deals with dynamic coating of the microchannels fabricated on poly(methyl methacrylate) (PMMA) chips andDNAseparation by
microchip electrophoresis (MCE). After testing a number of polymers, including 2-hydroxyethyl cellulose, hydroxypropylmethyl cellulose,
different sizes of poly(ethylene oxide) (PEO), and poly(vinyl pyrrolidone) (PVP), we found that coating of the PMMA microchannels
with PEO(Mr = 6.0 × 105 g/mol) on the first layer is essential to minimize the interaction of DNA with PMMA surface. To achieve high efficiency, multilayer coating of PMMA chips with PEO, PVP, and PEO containing gold nanoparticles [PEO(GNP)] is important. A 2- (PEO–PVP)–PEO(GNP) PMMA chip, which was repeatedly coated with 1.0% PEO and 5.0% PVP twice, and then coated with 0.75% PEO(GNP) each for 30 min, provided a high efficiency (up to 1.7 × 106 plates/m) for the separation of DNA markers V (pBR 322/HaeIII digest) and VI (pBR 328/BglI digest and pBR 328/HinfI digest) when using 0.75% PEO(GNP).With such a high efficiency, we demonstrated the separation of hsp65 gene fragments of Mycobacterium HaeIII digests by MCE within 90 s. The advantages of this approach to DNA analysis include ease of filling the microchannel with 0.75% PEO(GNP), rapidity, and reproducibility.
關聯: J. Chromatogr. A, 1073, 191-199
显示于类别:[化學系] 期刊論文

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