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http://ir.ncue.edu.tw/ir/handle/987654321/1553
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| Title: | Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering |
| Authors: | Huang, Y.-F.;Lin, Y.-W.;Lin, Z. -H.;Chang, H.-T. |
| Contributors: | 化學系 |
| Keywords: | Aptamer
Gold nanoparticles
Breast cancer cells
Imaging
Proliferation
Nanomedicine |
| Date: | 2009
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| Issue Date: | 2010-11-10T06:50:11Z
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| Abstract: | In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These
results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells
led to the generation of an intense scattered light upon
photo-illumination; this phenomenon allows the differentiation
of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231
cancer cells, but not H184B5F5/M10 cells. |
| Relation: | J. Nano. Res., 11, 775-783 |
| Appears in Collections: | [化學系] 期刊論文
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