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NCUEIR > College of Science > Department of Chemistry > Periodical Articles > Item 987654321/1554

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/1554

Title:	Using electrospray ionization mass spectrometry to explore the interactions among polythymine oligonucleotides, ethidium bromide, and mercury ions
Authors:	Chiang, C.-K.;Lin, Y.-W.;Hu, C.-C.;Chang, H.-T.
Contributors:	化學系
Date:	2009
Issue Date:	2010-11-10T06:50:58Z
Abstract:	We have used electrospray ionization mass spectrometry (ESI-MS) and fluorescence and circular dichroism (CD) spectroscopy to explore the binding of ethidium bromide (EthBr) to non-self-complementary polythymine (polyT) strands in the absence and presence of Hg2 ions. In the gas phase, ESI-MS revealed that Hg2 ions have greater affinity, through T–Hg2 –T coordination, toward polyT strands than do other metal ions. These findings are consistent with our fluorescence and CD results obtained in solution; they revealed that more T33–EthBr–Hg2 complexes existed upon increasing the concentrations of Hg2 ions (from 0 to 50 M). Surprisingly, the ESI-MS data indicated that the Hg2 concentration dependence of the interaction between T33 and EthBr is biphasic. Our ESI-MS data revealed that the T33–EthBr–Hg2 complexes formed with various stoichiometries depending on their relative concentrations of the components and the length of the DNA strand. When the concentrations of T33/EthBr/Hg2 were 5/5/2.5 M and 5/10/7.5 M, 1:1:1 and 1:1:2 T33–EthBr–Hg2 complexes were predominantly formed, respectively. Thus, Hg2 -induced DNA conformational changes clearly affect the interactions between DNA and EthBr. (J Am Soc Mass Spectrom 2009, 20, 1834 –1840) © 2009 American Society for Mass Spectrometry.
Relation:	J. Am. Soc. Mass Spectrom. 20, 1834-1840
Appears in Collections:	[Department of Chemistry] Periodical Articles

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