National Changhua University of Education Institutional Repository : Item 987654321/1554
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6498/11670
造访人次 : 25982752      在线人数 : 206
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻


题名: Using electrospray ionization mass spectrometry to explore the interactions among polythymine oligonucleotides, ethidium bromide, and mercury ions
作者: Chiang, C.-K.;Lin, Y.-W.;Hu, C.-C.;Chang, H.-T.
贡献者: 化學系
日期: 2009
上传时间: 2010-11-10T06:50:58Z
摘要: We have used electrospray ionization mass spectrometry (ESI-MS) and fluorescence and circular dichroism (CD) spectroscopy to explore the binding of ethidium bromide (EthBr) to non-self-complementary polythymine (polyT) strands in the absence and presence of Hg2 ions. In the gas phase, ESI-MS revealed that Hg2 ions have greater affinity, through T–Hg2 –T coordination, toward polyT strands than do other metal ions. These findings are consistent with our fluorescence and CD results obtained in solution; they revealed that more T33–EthBr–Hg2 complexes existed upon increasing the concentrations of Hg2 ions (from 0 to 50 M). Surprisingly, the ESI-MS data indicated that the Hg2 concentration
dependence of the interaction between T33 and EthBr is biphasic. Our ESI-MS data revealed that the T33–EthBr–Hg2 complexes formed with various stoichiometries depending on their relative concentrations of the components and the length of the DNA strand. When the concentrations of T33/EthBr/Hg2 were 5/5/2.5 M and 5/10/7.5 M, 1:1:1 and
1:1:2 T33–EthBr–Hg2 complexes were predominantly formed, respectively. Thus, Hg2 -induced DNA conformational changes clearly affect the interactions between DNA and EthBr. (J Am Soc Mass Spectrom 2009, 20, 1834 –1840) © 2009 American Society for Mass Spectrometry.
關聯: J. Am. Soc. Mass Spectrom. 20, 1834-1840
显示于类别:[化學系] 期刊論文





DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈