National Changhua University of Education Institutional Repository : Item 987654321/15632
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6507/11669
造访人次 : 29946886      在线人数 : 600
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/15632

题名: Synergistic Antitumor Effect of Curcumin and Dinitrosyl Iron Complexes for Against Melanoma Cells
作者: Wen, Yu-Der;Ho, Yun-Lung;Shiau, Rong-Jen;Yeh, Jung-Kai;Wu, Jheng-Yu;Wang, Wei-Lung;Chiou, Show-Jen
贡献者: 生物學系
日期: 2010-02
上传时间: 2013-03-12T01:49:28Z
出版者: Elsevier
摘要: The aim of this study was to examine whether combining curcumin, a chemoprevention agent, and dinitrosyl iron complexes (DNICs) would have a synergistic cytotoxic effect on mouse melanoma B16-F10 cells in vitro. Three synthesized DNICs-[PPN] [(NO)2Fe(SCH2CONHCH3)2] (NC01), [PPN] [(NO)2Fe(SCH2CON(CH3)2)2] (NC02), and [Na][(NO)2Fe(SCH2CON(CH3)2)2] (NC03) were tested in this study. In vitro DNA cleavage assay showed all three DNICs could cause plasmid DNA damage through releasing NO under UV irradiation. The cytotoxicity assay demonstrated these DNICs were toxic to B16-F10 cells in vitro, and the estimated values of LD50 (24 h of incubation) of NC01 and NC02 were 1 μM, while the values of LD50 of NC03 was 200 μM. No synergistic cytotoxicity effect was noted in the treatments of the combinations of curcumin and DNICs. On the contrary, in the presence of NC03, the toxicity of curcumin was reduced. Using UV–Visible spectroscopy and fluorescence microscopy, we found NC03 might interact with curcumin and reduce the accumulation of curcumin in cells. Further experiments using the pretreatment of curcumin for 4 h followed by the treatment of NC03 showed the synergistic cytotoxic effect, while, the pretreatment of NC03 followed by the treatment of curcumin did not have any effect. This study provides the basis for further investigation on the effects of combinations of curcumin and other NO donors.
關聯: Journal of Organometallic Chemistry, 695(3): 352-359
显示于类别:[生物學系] 期刊論文

文件中的档案:

档案 大小格式浏览次数
index.html0KbHTML749检视/开启


在NCUEIR中所有的数据项都受到原著作权保护.

 

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈