National Changhua University of Education Institutional Repository : Item 987654321/15804
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題名: Exocytosis of a Single Bovine Adrenal Chromaffin Cell: The Electrical and Morphological Studies
作者: Tsai, Chia-Chang;Yang, Chih-Cheng;Shih, Po-Yuan;Wu, Cen-Shawn;Chen, Chii-Dong;Pan, Chien-Yuan;Chen, Yit-Tsong
貢獻者: 物理學系
日期: 2008
上傳時間: 2013-03-12T04:06:33Z
出版者: American Chemical Society
摘要: Exocytosis of a single bovine adrenal chromaffin cell, triggered by histamine stimulation, was investigated via the electric responses detected with single-walled carbon-nanotube field-effect transistors (SWCNT-FET) and the morphological changes acquired by atomic force microscopy (AFM). Secretion of chromogranin A (CgA), stored in the vesicles of a single chromaffin cell, can be monitored in situ by the antibody against CgA (CgA-antibody) functionalized on the SWCNT-FET devices. The SWCNT-FET can further discriminate the amount of released CgA with different levels of histamine stimulations. The AFM morphological studies on a chromaffin cell indicate that the depression structures on the cell surface, caused by the histamine-evoked exocytotic fusion pores, appeared much more frequently than those without histamine stimulation or with the pretreatment of mepyramine before histamine stimulation. The vesicle diameters are about 50 nm calculated from the obtained three-dimensional AFM images. In comparison, the fusion pores of chromaffin cells stimulated by high-K+ buffer solution were also investigated to have a wider-ranging distribution of vesicle diameters of 60−260 nm. This work demonstrates that the combination of novel techniques, SWCNT-FET and AFM, can provide further insights into the fundamental properties of exocytosis in neuroendocrine cells.
關聯: Journal of Physical Chemistry B, 112(30): 9165-9173
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