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| 題名: | Cloning and Expression of a cDNA Coding for Catalase from Zebrafish (Danio rerio) |
| 作者: | Ken, Chuian-Fu;Lin, Chi-Tsai;Wu, Jen-Leih;Shaw, Jei-Fu |
| 貢獻者: | 生物學系 |
| 關鍵詞: | Zebrafish;Danio rerio;Molecular cloning;cDNA;Catalase;Expression;Escherichia coli;RACE-PCR;pET-20b(+) |
| 日期: | 2000-06
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| 上傳時間: | 2013-06-05T08:48:08Z
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| 出版者: | American Chemical Society |
| 摘要: | A full-length complementary DNA (cDNA) clone encoding a catalase was amplified by the rapid amplication of cDNA ends-polymerase chain reaction (RACE-PCR) technique from zebra fish (Danio rerio) mRNA. Nucleotide sequence analysis of this cDNA clone revealed that it comprised a complete open reading frame coding for 526 amino acid residues and that it had a molecular mass of 59 654 Da. The deduced amino acid sequence showed high similarity with the sequences of catalase from swine (86.9%), mouse (85.8%), rat (85%), human (83.7%), fruit fly (75.6%), nematode (71.1%), and yeast (58.6%). The amino acid residues for secondary structures are apparently conserved as they are present in other mammal species. Furthermore, the coding region of zebrafish catalase was introduced into an expression vector, pET-20b(+), and transformed into Escherichia coli expression host BL21(DE3)pLysS. A 60-kDa active catalase protein was expressed and detected by Coomassie blue staining as well as activity staining on polyacrylamide gel followed electrophoresis. |
| 關聯: | Journal of Agricultural and Food Chemistry, 48(6): 2092-2096 |
| 顯示於類別: | [生物學系] 期刊論文
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