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NCUEIR > College of Science > Department of Biology > Periodical Articles > Item 987654321/16769

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/16769

Title:	Characterization of Copper/Zinc-Superoxide Dismutase from Pagrus major cDNA and Enzyme Stability
Authors:	Ken, Chuian-Fu;Weng, De-Feng;Duan, Kow-Jen;Lin, Chi-Tsai
Contributors:	生物學系
Keywords:	Pagrus major;Expression;Escherichia coli;PCR;pET-20b(+)
Date:	2002-02
Issue Date:	2013-06-05T08:48:08Z
Publisher:	American Chemical Society
Abstract:	A full-length cDNA of 794 bp encoding a putative copper/zinc-superoxide dismutase (Cu/Zn-SOD) from Pagrus major was cloned by the PCR approach. Nucleotide sequence analysis of this cDNA clone revealed that it comprises a complete open reading frame coding for 154 amino acid residues. The deduced amino acid sequence showed high similarity (53−91%) with the sequences of Cu/Zn-SOD from other species. Computer analysis of the residues required for coordinating copper (His-47, 49, 64, and 121) and zinc (His-64, 72, 81, and Asp-84), as well as the two cysteines (58 and 147) that form a single disulfide bond, were well conserved among all reported Cu/Zn-SOD sequences. To further characterize the Pagrus major Cu/Zn-SOD, the coding region was subcloned into an expression vector, pET-20b(+), and transformed into Escherichia coli BL21(DE3). The expression of the Cu/Zn-SOD was confirmed by enzyme activity stained on a native-gel and purified by Ni2+-nitrilotriacetic acid Sepharose superflow. Dimer was the major form of the enzyme in equilibrium. The dimerization of the enzyme was inhibited under acidic pH (below 4.0 or higher than 10.0). The half-life was 8.6 min and the inactivation rate constant (kd) was 9.69 × 10-2 min-1 at 70 °C. The enzyme activity was not significantly affected under 4% SDS or 0.5 M imidazole. The enzyme was resistant to proteolysis by both trypsin and chymotrypsin.
Relation:	Journal of Agricultural and Food Chemistry, 50(4): 784-789
Appears in Collections:	[Department of Biology] Periodical Articles

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