English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 6507/11669
造訪人次 : 30066954      線上人數 : 641
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 進階搜尋

請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/16770

題名: Characterization of Fish Cu/Zn–Superoxide Dismutase and Its Protection from Oxidative Stress
作者: Ken, Chuian-Fu;Lin, Chi-Tsai;Shaw, Jei-Fu;Wu, Jen-Leih
貢獻者: 生物學系
關鍵詞: Cu/Zn superoxide dismutase;Overexpression;Zebrafish;Paraquat;Escherichia coli
日期: 2003-04
上傳時間: 2013-06-05T08:48:10Z
出版者: Springer-Verlag
摘要: Copper/zinc superoxide dismutase was cloned from the zebrafish (Danio rerio). The full coding region of the zebrafish superoxide dismutase (ZSOD) complementary DNA was ligated with pET-20b(+) and successfully expressed in Escherichia coli strain AD494(DE3)pLysS. The active enzyme was purified by His tagging. The ZSOD yield was 6 mg from 0.2 L of E. coli culture, and the specific activity was 2000 U/mg as assayed using a RANSOD kit. The enzyme stability was characterized by reaction to temperature, pH, and detergent treatment. The results showed enzyme activity was still active after heat treatment at 70�C for 10 minutes, resistant to pH treatment from 2.3 to 12, and resistant to treatment with sodium dodecyl sulfate (SDS) under 4%. In addition, the recombinant ZSOD was used to protect fish from 100 ppm of paraquat-induced oxidative injury by soaking fish larva in 55 �g/ml SOD enzyme. The results were significant.
關聯: Marine Biotechnology, 5(2): 167-173
顯示於類別:[生物學系] 期刊論文

文件中的檔案:

檔案 大小格式瀏覽次數
index.html0KbHTML526檢視/開啟


在NCUEIR中所有的資料項目都受到原著作權保護.

 

DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋