National Changhua University of Education Institutional Repository : Item 987654321/16773
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 6491/11663
造访人次 : 24748194      在线人数 : 42
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 进阶搜寻

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/16773

题名: Unusual Stability of Manganese Superoxide Dismutase from a New Species, Tatumella Ptyseos ct: Its Gene Structure, Expression, and Enzyme Properties
作者: Ken, Chuian-Fu;Lee, Chuing-Chi;Duan, Kow-Jen;Lin, Chi-Tsai
贡献者: 生物學系
关键词: Tatumella ptyseos ct;Manganese superoxide dismutase;Cosmetic;Expression;His-tag purification
日期: 2005-03
上传时间: 2013-06-05T08:48:14Z
出版者: Elsevier
摘要: A genomic DNA of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase (Mn-SOD) from Tatumella ptyseos ct was cloned. Sequence analysis of this new gene revealed that it translates 205 amino acid residues. The deduced amino acid sequence showed variable identities (41–91%) with sequences of Mn-SODs from other species. The residues required to coordinate the single trivalent manganese ion and the 11 residues putatively involved in the active center are conserved as they are in other reported Mn-SODs. In addition, the gene was introduced into the expression vector, pET-20b(+), and transformed in Escherichia coli BL21(DE3). The Mn-SOD was purified by a His-tag technique. The yield was 0.9 mg from 0.5 L of culture. The specific activity was 6540 U/mg. A dimer is the major form of the enzyme in equilibrium. The half-life of dimer is approximately 50 min and its thermal inactivation rate constant kd was 0.015 min 1 at 80 C. The dimerization of the enzyme was inhibited under an acidic pH (below 4.0), or in the presence of SDS (above 1%) or imidazole (above 0.5 M), whereas it was not affected under an alkaline pH (above 9.0). Furthermore, the dimeric enzyme was much more resistant to proteolytic attack after 3 h of incubation at 37 C with trypsin and chymotrypsin. This unusually stable enzyme can be used as cosmetic to the protection of skin against the unaesthetic effects caused by free radicals.
關聯: Protein Expression and Purification, 40(1): 42-50
显示于类别:[生物學系] 期刊論文

文件中的档案:

档案 大小格式浏览次数
index.html0KbHTML493检视/开启


在NCUEIR中所有的数据项都受到原著作权保护.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈