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Please use this identifier to cite or link to this item:
http://ir.ncue.edu.tw/ir/handle/987654321/16774
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| Title: | Biochemical Characterization of a Novel 2-Cys Peroxiredoxin from Antrodia Camphorata |
| Authors: | Huang, Jenq-Kuen;Ken, Chuian-Fu;Huang, Hui-Ming;Lin, Chi-Tsai |
| Contributors: | 生物學系 |
| Keywords: | Mushroom;Antrodia camphorata;Expression;2-Cys Peroxiredoxin (2-Cys Prx) |
| Date: | 2007-02
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| Issue Date: | 2013-06-05T08:48:15Z
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| Publisher: | SpringLink |
| Abstract: | Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified
enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60�C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin. |
| Relation: | Appl Microbiol Biotechnol, 74(1): 84-92 |
| Appears in Collections: | [生物學系] 期刊論文
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