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NCUEIR > College of Science > Department of Biology > Periodical Articles > Item 987654321/16774

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/16774

Title:	Biochemical Characterization of a Novel 2-Cys Peroxiredoxin from Antrodia Camphorata
Authors:	Huang, Jenq-Kuen;Ken, Chuian-Fu;Huang, Hui-Ming;Lin, Chi-Tsai
Contributors:	生物學系
Keywords:	Mushroom;Antrodia camphorata;Expression;2-Cys Peroxiredoxin (2-Cys Prx)
Date:	2007-02
Issue Date:	2013-06-05T08:48:15Z
Publisher:	SpringLink
Abstract:	Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60�C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
Relation:	Appl Microbiol Biotechnol, 74(1): 84-92
Appears in Collections:	[Department of Biology] Periodical Articles

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