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Title: Biochemical Characterization of a Novel 2-Cys Peroxiredoxin from Antrodia Camphorata
Authors: Huang, Jenq-Kuen;Ken, Chuian-Fu;Huang, Hui-Ming;Lin, Chi-Tsai
Contributors: 生物學系
Keywords: Mushroom;Antrodia camphorata;Expression;2-Cys Peroxiredoxin (2-Cys Prx)
Date: 2007-02
Issue Date: 2013-06-05T08:48:15Z
Publisher: SpringLink
Abstract: Peroxiredoxins (Prxs) play important roles in antioxidation and cell signaling. A gene encoding a novel 2-Cys Prx was identified based on sequence homology in an expressed sequence tag database of the Antrodia camphorata, a medicinal mushroom found only in Taiwan. The 2-Cys Prx cDNA (940 bp) encodes a protein of 188 amino acid residues with calculated molecular mass of 20,965 Da and a pI of 5.89. The coding region was subcloned into pAVD10, transformed into Escherichia coli, and expressed as a His-tagged fusion protein. The purified
enzyme was characterized under various conditions. The Prx retained 68% activity after being heated at 60�C for 2 min. It was stable under a broad pH range from 5 to 11. The enzyme activity was slightly decreased in the presence of 1% sodium dodecyl sulfate. The enzyme was somewhat susceptible to chymotrypsin treatment but resistant to digestion by trypsin.
Relation: Appl Microbiol Biotechnol, 74(1): 84-92
Appears in Collections:[Department of Biology] Periodical Articles

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