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题名: Putative Phospholipid Hydroperoxide Glutathione Peroxidase from Antrodia Camphorate
作者: Chen, Hsueh-Tai;Lin, Choa-Yi;Ken, Chuian-Fu;Wen, Lisa;Lin, Chi-Tsai
贡献者: 生物學系
关键词: Antrodia camphorata;Antioxidation;Phospholipid hydroperoxide glutathione peroxidase (PHGPx);Taiwanofungus
日期: 2009-07
上传时间: 2013-06-05T08:48:21Z
出版者: Elsevier
摘要: Glutathione Peroxidases (GPxs) play important roles in antioxidation. A cDNA (Ac-PHGPx, 764 bp) encoding a putative phospholipid hydroperoxide glutathione peroxidase (PHGPx) from Antrodia camphorata has been cloned. The deduced amino acid sequence is conserved among the reported GPxs. To characterize the Ac-PHGPx, the coding region was subcloned into pYEX-S1 and transformed into Saccharomyces cerevisiae. The recombinant 6His-tagged Ac-PHGPx was expressed and purified by Ni2+-nitrilotriacetic acid Sepharose. The purified enzyme showed a predominant band with molecular mass of ∼18 kDa on 12% SDS–PAGE. The enzyme retained 50% activity at 60 °C for 8 min. The enzyme was most active at pH 9. The enzyme showed 42% activity after incubation with trypsin at 37 °C for 40 min. In addition, the ability of Ac-PHGPx to protect intact supercoiled plasmid DNA from OH induced nicking was demonstrated.
關聯: Food Chemistry, 115(2): 476-482
显示于类别:[生物學系] 期刊論文


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