National Changhua University of Education Institutional Repository : Item 987654321/16785

English | 正體中文 | 简体中文 | Items with full text/Total items : 6507/11669
Visitors : 30019898 Online Users : 342

RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.

Scope Adv. Search

Login ‧ Upload ‧ Help ‧ About ‧ Administer

NCUEIR > College of Science > Department of Biology > Periodical Articles > Item 987654321/16785

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/16785

Title:	Taiwanofungus Camphorata Nitroreductase: cDNA Cloning and Biochemical Characterisation
Authors:	Chen, Chih-Chen;Ken, Chuian-Fu;Wen, Lisa;Chang, Ching-Fong;Lin, Chi-Tsai
Contributors:	生物學系
Keywords:	Nitroreductase;Taiwanofungus camphorata;Three-dimension structural model (3-D structural model)
Date:	2012-12
Issue Date:	2013-06-05T08:48:29Z
Publisher:	Elsevier
Abstract:	Nitroreductases (Nrs) play important roles in redox system via NADPH or NADH as a reductant. A TcNr cDNA encoding a putative Nr was cloned from Taiwanofungus camphorata. A 3-D structural model of the TcNr has been created based on the known structure of BcNr (Bacillus cereus). To characterise the TcNr, the coding region was subcloned into an expression vector and transformed into Escherichia coli. The recombinant His6-tagged TcNr was purified by Ni affinity chromatography. The purified enzyme showed a single band at molecular mass of approximately 25 kDa on 12% sodium dodecyl sulphate–polyacrylamide gel electrophoresis. The enzyme exhibited Nr activity via ferricyanide assay. The Michaelis constant (KM) value for ferricyanide was 0.86 mM. The enzyme’s half-life of deactivation at 45 °C was 12.3 min. The enzyme was most active at pH 6. The enzyme’s preferred substrate is 1-chloro-2, 4-dinitrobenzene.
Relation:	Food Chemistry, 135(4): 2708-2713
Appears in Collections:	[Department of Biology] Periodical Articles

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	766	View/Open