National Changhua University of Education Institutional Repository : Item 987654321/1839
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 6507/11669
造訪人次 : 29718643      線上人數 : 404
RC Version 3.2 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 進階搜尋

請使用永久網址來引用或連結此文件: http://ir.ncue.edu.tw/ir/handle/987654321/1839

題名: Synergistic Antitumor Effect of Curcumin and Dinitrosyl Iron Complexes for Against Melanoma Cells
作者: Wen YD;Ho YL;Shiau RJ;Yeh JK;Wu JU;Wang WL;Chiou SJ.
貢獻者: 生物學系
關鍵詞: Curcumin
Cytotoxicity
Dinitrosyl iron complexes
Melanoma
日期: 2010
上傳時間: 2010-11-15T07:08:50Z
摘要: The aim of this study was to examine whether combining curcumin, a chemoprevention agent, and dinitrosyl iron complexes (DNICs) would have a synergistic cytotoxic effect on mouse melanoma B16-F10 cells in vitro. Three synthesized DNICs-[PPN] [(NO)2Fe(SCH2CONHCH3)2] (NC01), [PPN] [(NO)2Fe(SCH2-CON(CH3)2)2] (NC02), and [Na][(NO)2Fe(SCH2CON(CH3)2)2] (NC03) were tested in this study. In vitro DNA cleavage assay showed all three DNICs could cause plasmid DNA damage through releasing NO under UV irradiation. The cytotoxicity assay demonstrated these DNICs were toxic to B16-F10 cells in vitro, and the estimated values of LD50 (24 h of incubation) of NC01 and NC02 were 1 lM, while the values of LD50 of NC03 was 200 lM. No synergistic cytotoxicity effect was noted in the treatments of the combinations of curcumin and DNICs. On the contrary, in the presence of NC03, the toxicity of curcumin was reduced. Using UV–Visible spectroscopy and fluorescence microscopy, we found NC03 might interact with curcumin and reduce the accumulation of curcumin in cells. Further experiments using the pretreatment of curcumin for 4 h followed by the treatment of NC03 showed the synergistic cytotoxic
effect, while, the pretreatment of NC03 followed by the treatment of curcumin did not have any effect. This study provides the basis for further investigation on the effects of combinations of curcumin and other NO donors.
關聯: Journal of Organometallic Chemistry, 695:352-359 (SCI)
顯示於類別:[生物學系] 期刊論文

文件中的檔案:

沒有與此文件相關的檔案.



在NCUEIR中所有的資料項目都受到原著作權保護.

 


DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋