National Changhua University of Education Institutional Repository : Item 987654321/8984

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NCUEIR > College of Science > biotech > Periodical Articles > Item 987654321/8984

Please use this identifier to cite or link to this item: http://ir.ncue.edu.tw/ir/handle/987654321/8984

Title:	Genetics of the Tryptophan Biosynthetic Pathway of the Procaryotic Endosymbiont (Buchnera) of the Aphid Schlechtendalia Chinensis
Authors:	Lai, C.Y.;P. Baumann;N.A. Moran
Contributors:	生物系
Keywords:	Schlechtendalia;Chinensis;Schizaphis;Graminum;Buchnera;Aphidicola;Endosymbiosis;Mutualism;tryptophan;Biosynthesis;TrpA;trpB;TrpC(F);trpD;TrpE;trpG;SohB
Date:	1995-02
Issue Date:	2011-05-16T07:38:56Z
Publisher:	Wiley-Blackwell
Abstract:	Two DNA fragments (3941 and 7152 base pairs) from the procaryotic endosymbiont (Buchnera) of the aphid Schlechtendalia chinensis were cloned and sequenced. The smaller fragment contained trpEG and the larger fragment contained trpDC(F)BA, genes coding for enzymes of the tryptophan biosynthetic pathway which convert chorismate to tryptophan. Both of these gene clusters were present as one copy on the endosymbiont chromosome and probably constitute two transcription units. The deduced amino acid sequences of the proteins was 51-61% identical to the corresponding proteins were previously studied in Buchnera of the aphid Schizaphis graminum. In this endosymbiont, trpEG is amplified and located on a plasmid, whereas, in the endosymbiont of S. chinensis, as in other eubacteria, trpEG occurs as a single copy on the bacterial chromosome. Implications of these findings for the evolution of this mutualistic association are discussed.
Relation:	Insect Molecular Biology, 4(1): 47-59
Appears in Collections:	[biotech] Periodical Articles

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