National Changhua University of Education Institutional Repository : Item 987654321/8984
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题名: Genetics of the Tryptophan Biosynthetic Pathway of the Procaryotic Endosymbiont (Buchnera) of the Aphid Schlechtendalia Chinensis
作者: Lai, C.Y.;P. Baumann;N.A. Moran
贡献者: 生物系
关键词: Schlechtendalia;Chinensis;Schizaphis;Graminum;Buchnera;Aphidicola;Endosymbiosis;Mutualism;tryptophan;Biosynthesis;TrpA;trpB;TrpC(F);trpD;TrpE;trpG;SohB
日期: 1995-02
上传时间: 2011-05-16T07:38:56Z
出版者: Wiley-Blackwell
摘要: Two DNA fragments (3941 and 7152 base pairs) from the procaryotic endosymbiont (Buchnera) of the aphid Schlechtendalia chinensis were cloned and sequenced. The smaller fragment contained trpEG and the larger fragment contained trpDC(F)BA, genes coding for enzymes of the tryptophan biosynthetic pathway which convert chorismate to tryptophan. Both of these gene clusters were present as one copy on the endosymbiont chromosome and probably constitute two transcription units. The deduced amino acid sequences of the proteins was 51-61% identical to the corresponding proteins were previously studied in Buchnera of the aphid Schizaphis graminum. In this endosymbiont, trpEG is amplified and located on a plasmid, whereas, in the endosymbiont of S. chinensis, as in other eubacteria, trpEG occurs as a single copy on the bacterial chromosome. Implications of these findings for the evolution of this mutualistic association are discussed.
關聯: Insect Molecular Biology, 4(1): 47-59
显示于类别:[生物技術研究所] 期刊論文

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